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Revision Date: Feb.11, 2010
ii. Do not store food in refrigerators or freezers where biological materials, chemicals
or radioactives are kept. All freezers and refrigerators in the laboratory must be
labeled to identify proper storage.
iii. Mouth pipetting is prohibited.
iv. Do not break, shear, or recap hypodermic needles, or remove hypodermic needles
from disposable syringes.
v. Do not place your head into a Biological Safety Cabinet.
vi. Do not sniff cultures and other samples.
vii. Other prohibited (unsafe) work practices specific to the laboratory:
5. Engineering controls:
BSL-2 laboratories:
a. All procedures involving the potential aerosolization of infectious materials are conducted
within a certified Biological Safety Cabinet or other physical containment device.
i. Specify other available physical containment devices at your laboratory locations:
b. An eyewash is available.
c. Laboratory doors are self-closing.
d. The laboratory floors and walls can be easily cleaned and decontaminated.
e. Carpets and rugs are not permitted. Seams, floors, walls, and ceiling surfaces are sealed.
f. Furniture can be easily cleaned and decontaminated. Chairs are covered with non-porous
material than can be easily cleaned and decontaminated with appropriate disinfectant.
g. Spaces between benches, cabinets, and equipment are accessible for cleaning.
h. A sink for hand washing is available in the laboratory.
i. An autoclave capable of inactivating infectious waste is available or a vendor is used to
treat infectious waste.
6. Personal protective equipment (PPE):
a. Check PPE to be used when working with and near potential ATPs
Lab coat
Closed front gown/coverall
Single gloves
Double gloves
Goggles
Respirator
Shoe covers
Other:
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All individuals using a respirator must participate in the campus respiratory protection program, including medical clearance
and fit testing by Environmental Health and Safety.
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